玉米秸秆消化率全基因组关联分析

48h体外干物质消化率（48h in vitro dry matter digestibility, 48h IVDMD）是衡量青贮玉米品质的重要指标。为了初步探究玉米秸秆消化率的分子遗传机理,以341份玉米自交系为材料,于2018年在沈阳和通辽种植,收获后测定秸秆48h IVDMD。利用全基因组重测序获得的6 276 612个高质量SNPs进行全基因组关联分析,共检测到153个与玉米秸秆消化率显著相关的SNPs位点（P<1.0×10^-6）,4个SNPs显著水平在P<1.0×10^-8以上;共找到38个秸秆消化率的候选基因,主要涉及细胞生长发育、防御反应和信号转导等生物学功能。     

Cover crop evapotranspiration under semi-arid conditions using FAO dual crop coefficient method with water stress compensation

Cover cropping is a common agro-environmental tool for soil and groundwater protection. In water limited environments, knowledge about additional water extraction by cover crop plants compared to a bare soil is required for a sustainable management strategy. Estimates obtained by the FAO dual crop coefficient method, compared to water balance-based data of actual evapotranspiration, were used to assess the risk of soil water depletion by four cover crop species (phacelia, hairy vetch, rye, mustard) compared to a fallow control. A water stress compensation function was developed for this model to account for additional water uptake from deeper soil layers under dry conditions. The average deviation of modelled cumulative evapotranspiration from the measured values was 1.4% under wet conditions in 2004 and 6.7% under dry conditions in 2005. Water stress compensation was suggested for rye and mustard, improving substantially the model estimates. Dry conditions during full cover crop growth resulted in water losses exceeding fallow by a maximum of +15.8% for rye, while no substantially higher water losses to the atmosphere were found in case of evenly distributed rainfall during the plant vegetation period with evaporation and transpiration concentrated in the upper soil layer. Generally the potential of cover crop induced water storage depletion was limited due to the low evaporative demand when plants achieved maximum growth. These results in a transpiration efficiency being highest for phacelia (5.1 g m⁻² mm⁻¹) and vetch (5.4 g m⁻² mm⁻¹) and substantially lower for rye (2.9 g m⁻² mm⁻¹) and mustard (2.8 g m⁻² mm⁻¹). Taking into account total evapotranspiration losses, mustard performed substantially better. The integration of stress compensation into the FAO crop coefficient approach provided reliable estimates of water losses under dry conditions. Cover crop species reducing the high evaporation potential from a bare soil surface in late summer by a fast canopy coverage during early development stages were considered most suitable in a sustainable cover crop management for water limited environments.     

参考作物腾发量计算方法的适用性研究

选用5种方法,利用陕西6站的气象资料,计算了各站逐日ET0。并以FAO56 Penman-Monteith(P-M)法为标准,对其它方法进行评价。结果表明,在陕西6地区,5种方法计算的ET0变化趋势基本相同,但数值上有一定差异,所有的差异随ET0的增大而增大。Hargreaves法计算结果差异性较小,适用性较好;1948Penman和Priestley-Taylor二方法估值较FAO24 Penman法更接近P-M法的计算结果;缺气象资料时,Priestley-Taylor法可获得较好估值,且更适用于湿润地区;FAO24 Penman法也能获得较好结果,但其估值精度低于Priestley-Taylor法,一般不宜采用。同时分析了P-M法计算的ET0值和水面蒸发量之间的关系,为利用水面蒸发资料估算陕西6地区ET0值提供参考。     

Study on Proliferation and Apoptosis of EBL Cells Induced by P48 Protein of Mycoplasma bovis

The aim of this study was to investigate the effect of P48 protein on proliferation and apoptosis of embryonic bovine lung (EBL) cells.In this study,samples which co-incubated with P48 protein and EBL cells in different concentrations at different time points were collected,the proliferation rate of the cells was detected by MTT method,and the changes in the nuclear morphology of EBL cells were observed by DAPI staining method.Meanwhile,flow cytometry was used to detect the apoptosis rate of EBL cells induced by P48 protein.Real-time quantitative PCR was used to detect the changes in mRNA level of apoptotic marker genes,and Western blotting tested the Bax and Beclin-1 protein expressions.The results showed that under the condition of 72 h and 10 μg/mL of protein concentration treatment,P48 extremely significantly inhibited EBL cells proliferation (P<0.01),while 0.1 and 0.5 μg/mL protein concentration had no inhibitory effect (P>0.05).The nuclear morphology showed no significant change after protein induction for 12 h,but wrinkled and condensed at 24 h.The nucleus was fragmented,and a sprouted apoptotic body was appeared at 48 and 72 h.Apoptosis related genes expression showed no obvious increase at 2 and 12 h at mRNA level,but gradually increased at 24,48 and 72 h,and it showed a time-dependent manner.Accordingly,the expression of apoptosis marker proteins Bax and Beclin-1 significantly increased.Flow cytometry analysis showed that the apoptosis rate of EBL cells induced by P48 protein was 48.44%.In conclusion,P48 recombinant protein of Mycoplasmas bovis inhibited the proliferation of EBL cells and promoted their apoptosis,which provided reference for revealing the pathogenic mechanism of Mycoplasmas bovis.     

以糠醇为碳源液相浸渍法制备有序中孔炭分子筛

以纯硅MCM-48为模板、对甲苯磺酸为催化剂、糠醇为碳源成功制备了高度有序的中孔炭分子筛.结果表明,采用真空浸渍法能较好的将酸性催化剂对甲苯磺酸引入MCM-48孔道内.所用糠醇的体积用量为MCM-48孔体积的2.5倍为宜.产物用XRD、TEM、N2吸附等进行了表征,表明所制得的中孔炭具有规整的三维孔道结构,平均孔径 3.1 nm,孔容 0.96 m3/g,比表面积 1 513.6 m2/g.此外,还发现二次炭化有利于提高中孔炭的热稳定性.     

牛病毒性腹泻-黏膜病病毒噬菌体单链抗体库的构建和筛选

为筛选牛病毒性腹泻-黏膜病病毒(Bovine viral diarrhea virus,BVDV)gP48蛋白的单链抗体,本研究利用噬菌体展示技术构建了gP48蛋白单链抗体克隆文库,通过微孔筛选法对抗体库进行3轮富集淘筛,利用ELISA技术测定单链抗体的亲和力,对亲和力较高的克隆进行基因测序分析和结合活性测定,旨在获得gP48蛋白的单链抗体。结果表明:1)成功构建了库容量为4.3×10⁷的噬菌体单链抗体库,其重组率为83.3%,经三轮筛选对噬菌体抗体库进行富集,富集倍数为1.43×10⁴;2)优化了间接ELISA方法,筛选到3株与BVDV gP48蛋白具有高亲和力的单链抗体以及其基因序列,通过IgBLAST分析显示,3株单链抗体均为鼠源IgG。综上,从gP48蛋白的单链抗体库中筛选获得3个具有高亲和力的单链抗体,可用于后续BVDV检测方法的建立。     

Exotic species,fisheries and conservation of freshwater biodiversity in tropical Asia: the case of the Sepik River,Papua New Guinea

1. Development of effective conservation strategies for freshwater biodiversity must take account of the trade‐off between species preservation and human use of ecosystem goods and services. The latter cannot be prevented, and attempts to manage ecosystems that focus solely on maximizing biodiversity will fail. 2. A compromise position of management for ecosystem functioning and human livelihoods — rather than preservation of every species — will provide a better basis for biodiversity conservation. This has implications for the management of exotic species. 3. There are some situations, in lentic habitats in Sri Lanka, for example, where the establishment of exotics has increased fishery yields without apparent detriment to native biodiversity. The Sepik River in Papua New Guinea provides another illustration of potential compromises between human livelihoods and biodiversity conservation. 4. The Sepik supports a relatively unproductive fishery. Two fish stocking projects (in 1987–93 and 1993–97), representing a partnership between the Papua New Guinea Government and the United Nations (UNDP/FAO), led to the introduction of a suite of exotic fish into the Sepik. Species were selected on the basis of their potential to occupy niches not filled by native fish. Unfortunately, the outcomes of these introductions are poorly documented, although there is preliminary evidence both of increased human use of exotic fish as well as declines of some native species associated with the spread of exotics. 5. Better understanding of the results of the Sepik fish introductions is important, because the pressures of burgeoning human populations in most of tropical Asia make it impossible to preserve near‐pristine environments such as the Sepik. While attempts to conserve natural or near‐natural systems must remain a priority, there is a need to develop strategies for the management of damaged or degraded ecosystems, which may contain exotics, with the aim of maintaining ecosystem functioning and, if possible, maximizing the persistence of native biodiversity. Copyright © 2006 John Wiley & Sons, Ltd.     

Accessing genetic resources: international law establishes multilateral system

The International Treaty on Plant Genetic Resources for Food and Agriculture is rapidly gathering sufficient ratifications to become international law. Once it enters into force, it will define the rules for access and benefit-sharing associated with most genetic resources of major food crops. This paper explains how the new Multilateral System established by the Treaty will work, and points out a number of ambiguities and problems in the text. Despite these shortcomings, the Treaty provides the international community of researchers, plant breeders, and farmers with an opportunity to foster cooperation and further the conservation and use of plant genetic resources.     

FAO可持续土地资源管理综合规划指南及启示

土地资源可持续利用是基于可持续发展的核心内容——资源的可持续利用而形成的，指对人类生存所依赖的物质资源，在追求经济效益最大的同时，维持和改善土地资源的生产条件和环境基础。在当前社会主义市场经济体制下,可持续土地资源管理的任务主要依靠政府严格的土地利用规划事先调节和控制，引导、限制或鼓励人们的土地利用行为，保证土地资源的合理配置和可持续利用。该文试图在分析FAO的土地规划的概念、内容、体系、成功的关键因素、及编制的步骤和方法基础上，展开探讨并对完善我国土地利用规划提出了借鉴意义。     

花生中氟乐灵农药残留的优化气相色谱测定法

报道了采用气相色谱仪、电子捕获检测器测定花生中氟乐灵除草剂残留的分析方法。样品用甲醇浸渍提取,然后经二氯甲烷反萃取,浓缩后经弗罗里硅土柱层析净化。农药添加浓度分别为0.05、0.5、1mg/kg,回收率为75.6%～80.4%,最低检测浓度为2.21μg·kg-1。该方法能有效去除油脂干扰,高效准确,分离效果好,回收率高。